Comparison of the Real-Time PCR Tests for Factor V G1691A and Prothrombin G20210A with PCRRestriction Fragment Length Polymorphism and Direct Sequencing Tests
نویسندگان
چکیده
منابع مشابه
Frequency of factor V Leiden (G1691A) and prothrombin (G20210A) polymorphisms in Population of Kerman Province, Iran
Background & Aims:Thromboembolism is an acute cardiovascular disease that ranges from clinically unimportant to massive embolism. Both acquired and hereditary risk factors contribute to the disease. We aimed to determine the prevalence of two hereditary predisposing factor of the disease, prothrombin G20210A and factor V Leiden (G1691A) polymorphisms, in Kerman population.<br /...
متن کاملPrevalence of the Factor V G1691A and the Factor II/prothrombin G20210A gene polymorphisms among Tamilians.
We have investigated the prevalence of the Factor II G20210A and Factor V G1691A single nucleotide polymorphisms (SNPs) in a South Indian-Tamil Nadu population. The SNP genotyping was performed using a polymerase chain reaction (PCR)/restriction fragment length polymorphism analysis and by a recently FDA-approved LightCycler real-time PCR assay. Of 72 samples that were genotyped, 4 (5.5%) patie...
متن کاملRapid detection of prothrombotic mutations of prothrombin (G20210A), factor V (G1691A), and methylenetetrahydrofolate reductase (C677T) by real-time fluorescence PCR with the LightCycler.
storage of blood at room temperature is uncertain, but one possibility is that cleavage of the antigen occurs, releasing smaller fragments, each with multiple OVX1 epitopes. Different cutoffs at 7.2, 10.5, or 12.1 kilounits/L have been used in clinical studies (2, 4). The present findings, therefore, are clinically relevant because the assay range is narrow and the effect we have noted can caus...
متن کاملFactor V G1691A and prothrombin G20210A gene polymorphisms among Iranian patients with cerebral venous thrombosis
Objective: Cerebral venous thrombosis (CVT) is an important cause of stroke, especially in young adults, that has many predisposing factors. G20210A mutation in prothrombin gene (Factor II) and G1691A mutation in Factor V Leiden (FVL) are two common hereditary causes of CVT. This study aimed to study the rate of these mutations in patients with CVT from Fars Province in southern Iran. Methods: ...
متن کاملFactor V Leiden G1691A and prothrombin G20210A mutations among Palestinian patients with sickle cell disease
Background Vascular thrombosis is an important pathophysiological aspect of sickle cell disease (SCD). This study aimed to investigate the prevalence and clinical impact of factor V Leiden G1691A (FVL) and prothrombin G20210A mutations among Palestinian sickle cell disease (SCD) patients. Methods A total of 117 SCD patients, including 59 patients with sickle cell anemia (SS), 33 patients with...
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ژورنال
عنوان ژورنال: Journal of Laboratory Medicine and Quality Assurance
سال: 2015
ISSN: 1225-097X,2288-7261
DOI: 10.15263/jlmqa.2015.37.1.37